Hello,
One of the main challenges of this technology is specifically and accurately targeting the DNA sequence to be modified. Off-target effects may occur when the CRISPR-Cas9 system makes unintended cuts in the genome. This can lead to unwanted mutations and may pose a challenge in gene editing, affecting the safety and accuracy of existing therapies.
i need your concerns about this if possible
thank’s
Thank you for your engagement in my poster
here’s my answer:
crispr is not a human designed enzyme it’s A nature’s one made up by the bacteria it self ,the real key use of this technology is that the crispr cas-9 complex is programmed to targed only one sequence of the DNA that is made for it contains a cr RNA that guide it to its target and additionally In order for this enzyme to be able to perform the double-strand break (DBS), it must recognize a small sequence of (3-5) pb called PAM (protospacer adjacent motif) located at the beginning of the sequence of each the foreign DNA (the target ) and the spacer( the one is the cr RNA) , this about the potential mistake of recognition ,in the other hand , as you said dr yes there could be a high potential To mutate specially in the natural ways to repair. The break and that was a concern previously before developing a crispr derivative techniques such as prime editing witch kept the crispr’s ability to recognize a specific DNA sequence we chose and replaced the cutting sites with other loops that works on changing the bases form instead of cutting ( chemical interactions with the bases residues)
i hope i answered your question ,let me know if you hv any other ,thank you
Hello,
One of the main challenges of this technology is specifically and accurately targeting the DNA sequence to be modified. Off-target effects may occur when the CRISPR-Cas9 system makes unintended cuts in the genome. This can lead to unwanted mutations and may pose a challenge in gene editing, affecting the safety and accuracy of existing therapies.
i need your concerns about this if possible
thank’s
Thank you for your engagement in my poster
here’s my answer:
crispr is not a human designed enzyme it’s A nature’s one made up by the bacteria it self ,the real key use of this technology is that the crispr cas-9 complex is programmed to targed only one sequence of the DNA that is made for it contains a cr RNA that guide it to its target and additionally In order for this enzyme to be able to perform the double-strand break (DBS), it must recognize a small sequence of (3-5) pb called PAM (protospacer adjacent motif) located at the beginning of the sequence of each the foreign DNA (the target ) and the spacer( the one is the cr RNA) , this about the potential mistake of recognition ,in the other hand , as you said dr yes there could be a high potential To mutate specially in the natural ways to repair. The break and that was a concern previously before developing a crispr derivative techniques such as prime editing witch kept the crispr’s ability to recognize a specific DNA sequence we chose and replaced the cutting sites with other loops that works on changing the bases form instead of cutting ( chemical interactions with the bases residues)
i hope i answered your question ,let me know if you hv any other ,thank you